ctx tna2 astrocyte  (ATCC)

Journal: Discover Nano

Article Title: Solid lipid nanoparticles enhance piracetam’s neuroprotective action in streptozotocin-induced cognitive dysfunction

doi: 10.1186/s11671-026-04528-3

Figure Lengend Snippet: A In vitro release study of piracetam loaded solid lipid nanoparticle-2 (PSLN-2) in phosphate-buffered saline (PBS), pH 7.4. The study depicted a sustained release profile of piracetam from PSLN-2, whereas the burst release was observed for plain piracetam; B Percentage of inhibition of the DPPH scavenging activity by ascorbic acid, piracetam and PSLN-2 (IC 50 :18.92 ± 1.7, 27.8 ± 1.18, and 23.43 ± 0.21 μg/mL); C Percentage of inhibition of the ABTS scavenging activity by ascorbic acid, piracetam and PSLN-2 (IC 50 :13.34 ± 1.23, 2 3.9 ± 1.56, and 18.1 ± 1.04 μg/mL). All the data are represented as Mean ± SD where n = 3; D In vitro cytotoxicity study of PSLN-2 on CTX TNA2 astrocyte (CRL-2006) and HT22 mice neuronal hippocampus cell line (SCC129). Data showed biocompatible nature of experimental PSLN-2 with more than 85% cell viability at the highest tested concentrations. Data represented as Mean ± SD, where n = 3. Student’s t-test * p < 0.05

Article Snippet: CTX TNA2 astrocyte, type I cell line (CRL-2006) was collected from American Type Culture Collection, Manassas, VA.

Techniques: In Vitro, Saline, Inhibition, Activity Assay

Journal: Antioxidants

Article Title: Distinctive Effects of Fullerene C 60 and Fullerenol C 60 (OH) 24 Nanoparticles on Histological, Molecular and Behavioral Hallmarks of Alzheimer’s Disease in APPswe/PS1E9 Mice

doi: 10.3390/antiox14070834

Figure Lengend Snippet: Impact of fullerenol on cellular viability and reactive oxygen species (ROS) production in vitro. The percentage of viability in bEnd3, CTX TNA2, and BV2 cells is depicted in ( A ), while the level of ROS in bEnd3, CTX TNA2, and BV2 cells is shown in ( B ). Statistical significance is indicated by * p ≤ 0.05, in comparison to the control group and the group exposed solely to Aβ42.

Article Snippet: CTX TNA2 cells are type 1 astrocytes (CRL-2006, ATCC, Manassas, VA, USA), bEnd.3 cells are mouse brain endothelial cells (CRL-2299, ATCC, Manassas, VA, USA), and BV2 cells are mouse microglial cell lines (ABC-TC212S, AcceGen, Fairfield, NJ, USA).

Techniques: In Vitro, Comparison, Control

Journal: Theranostics

Article Title: LCN2 deficiency mitigates the neuroinflammatory damage following acute glaucoma

doi: 10.7150/thno.104752

Figure Lengend Snippet: Innate immune cell response after OGD/R injury. A : Diagram of OGD/R in vitro model. B : Western blotting showed the changes in MHC-II expression in BV2 microglia following OGD/R. C : Bar graphs depicted the fold of ODV of MHC-II in BV2 microglia (n = 4). D : Western blotting showed the changes in MHC-II expression in RAW264.7 macrophages following OGD/R. E : Bar graphs depicted the fold of ODV of MHC-II in RAW264.7 macrophages (n = 4). F : Western blotting showed the changes in C3 expression in CTX TNA2 astrocytes following OGD/R. G : Bar graphs depicted the fold of ODV of C3 in CTX TNA2 astrocytes (n = 5). Data in C, E, G were represented as mean ± SD; *** p < 0.001, **** p < 0.0001 (compared with the control group using one-way analysis of variance).

Article Snippet: BV2 microglia, RAW264.7 macrophages and CTX TNA2 astrocytes were obtained from the ATCC.

Techniques: In Vitro, Western Blot, Expressing, Control